DOTAP is an effective cationic lipid, originally utilized in commercial products as a gene transfection reagent. It is also applicable as a pharmaceutical excipient in liposomal formulations. Its notable application is EndoTAG-1, a cationic liposomal formulation of paclitaxel. DOTAP imparts a positive charge to EndoTAG-1, allowing it to selectively adhere to tumor endothelial cells (TECs) with negative charges. This selective adsorption facilitates targeted attack on tumor neovasculature without harming healthy vessels, effectively inhibiting tumor growth.
| Product Name | (2,3-Dioleoyloxy-propyl)-trimethylammonium -chloride (For Injection)，DOTAP |
| Chemical Name | N-[1-(2,3-DIOLEYLOXY)PROPYL]-N,N,N-TRIMETHYLAMMONIUM |
| CAS No. | 132172-61-3 |
| NMPA CDE registration no. | / |
| USFDA DMF No. | / |
| Quality grade | Injection |
| Quality standards | Manufacturer's standard |
| Molecular Formula | C42H80ClNO4 |
| Applications and examples | The polar headgroup of the cationic lipid DOTAP can stably bind to negatively charged DNA molecules through electrostatic interactions, forming a stable complex that enables effective gene transfection. This transfection reagent is very gentle, avoiding the cytotoxicity associated with other transfection methods, and has higher transfection efficiency compared to other lipid-based transfection reagents. Therefore, DOTAP can be used as a commercial transfection reagent. |
| Product code | O02002 |
| Package sizes | 1g, 10g, 50g |
| Storage condition | This product should be transported and stored in a sealed and light-protected container at a temperature of −20 ± 5°C. It should be kept away from strong oxidizing agents. Under proper use and storage conditions, it is valid for three years, with retesting conducted annually. |
High transfection efficiency: As a gene transfection reagent in commercial products, DOTAP offers a significantly gentler transfection process. Compared to other lipid-based transfection agents, it helps to mitigate cytotoxic effects and ensures a higher transfection efficiency.
Targeted Delivery: DOTAP can serve as a supplementary component in selective organ targeting (SORT) lipid nanoparticles (LNPs). Incorporating 10%-15% DOTAP in LNPs facilitates targeted drug delivery to the spleen, while increasing the DOTAP content to 50% achieves optimal lung delivery efficiency.
DOTAP is biologically inert, biodegradable, non-toxic, and non-immunogenic. Its polar head stably binds to negatively charged DNA molecules through electrostatic interactions, forming stable complexes spontaneously that enter cells and deliver nucleic acids into the cytoplasm without causing damage to the organism. It is therefore often used as a vector for eukaryotic transfection reagents and DNA vaccines.
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