As a zwitterionic sulfonic acid buffering agent, HEPES exhibits excellent buffering capacity within the pH range of 6.8 to 8.2 and can maintain a stable pH for an extended period. It is widely utilized in the preparation of liposomes, antibody drugs, nucleic acid drugs, as well as vaccines. Additionally, HEPES is frequently employed as an additive in cosmetics.
NMPA CDE registration no.: F20220000151
USFDA DMF no.: /
| Product Name | 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid；HEPES |
| Chemical Name | N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid |
| CAS No. | 7365-45-9 |
| NMPA CDE registration no. | F20220000151 |
| USFDA DMF No. | / |
| Quality grade | Injection |
| Quality standards | Conforms with USP, EP, JP, ChP standards |
| Molecular Formula | C8H18N2O4S |
| Applications and examples | It is widely used in cell culture, IVD test kits, cosmetics, biomedicines, etc. |
| Product code | A04002 |
| Package sizes | 1kg |
| Storage condition | Protected from light. It is valid for three years when used and stored properly. |
HEPES is a non-ionic amphoteric buffer with a pH range of 6.8 - 8.2 and a good capacity in the pH range of 7.2 - 7.4. It is widely used in cell culture, IVD detection kits, DNA/RNA extraction kits, PCR diagnostic kits, biomedicine and other fields.
HEPES buffer has no toxic effect on cells, and can control a constant pH range for a long time. It is often used in the buffer system of cell culture. The common working concentration is 10-25mM. Generally, the medium containing 20mmol/L of HEPES can achieve the buffer capacity. . Compared with bicarbonate buffer, HEPES showed better pH control ability in tissue or organ culture. It is also used as a buffer for quantitative and selective determination of antigen-antibody reactions.
One of the applications of HEPES is Onivyde. In 2015, Onivyde was approved by the U.S. Food and Drug Administration (FDA) for new drug marketing. In October 2016, Onivyde received new drug marketing approval from the European Medicines Agency (EMA). In April 2022, Onivyde was officially approved by China's National Medical Products Administration (NMPA) for the treatment of metastatic pancreatic cancer.
HEPES has excellent water solubility, weak cell permeability, and stable chemical properties. Manufactured in GMP-compliant facilities, our HEPES boasts low endotoxin levels, meet the quality standards specified in the Chinese Pharmacopoeia (ChP), United States Pharmacopeia (USP), Japanese Pharmacopoeia (JP), and European Pharmacopoeia (EP), and is readily available in stock. Most importantly, it enables NMPA/FDA registration submission.
In the absence of carbon dioxide, HEPES is capable of adjusting the pH by absorbing or releasing hydrogen ions, thus maintaining cellular stability.
If you have any questions, please don't hesitate to contact us. We're always here to help with Pharmaceutical Excipients for your business. We can provide products ranging from grams to tons. AVT is able to produce and even register the product in US FDA according to your special needs.
EN
jp 
ko 
fr 
es 
ru 
ms 
id 
bn 
