The molecular formula of HEPES is C8H18N2O4S. Its structure contains both an acidic sulfonic acid group (-SO3H) and two basic tertiary amine nitrogens, so it is amphoteric and can be used to prepare zwitterionic buffers. The buffer solution generally consists of a certain amount of HEPES dissolved in distilled water, and then sodium hydroxide is used to adjust the pH to an appropriate value. Its pH buffer range is generally 6.8~8.2.
NMPA CDE registration no.: F20220000151
USFDA DMF no.: /
| Product Name | 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid；HEPES |
| Chemical Name | N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid |
| CAS No. | 7365-45-9 |
| NMPA CDE registration no. | F20220000151 |
| USFDA DMF No. | / |
| Quality grade | Pharmaceutical |
| Quality standards | Conforms with USP, EP, JP, ChP standards |
| Molecular Formula | C8H18N2O4S |
| Applications and examples | It is widely used in cell culture, IVD test kits, cosmetics, biomedicines, etc. |
| Product code | A04001 |
| Package sizes | 1kg |
| Storage condition | Protected from light. It is valid for three years when used and stored properly. |
HEPES (pharmaceutical grade) has gained widespread attention and application in the field of biologicals. For example, it is used in open cell growth, long-term observation of cells, and RNA or DNA extraction kits.
Produced in accordance with GMP guidelines and with strict microbial and endotoxin limits control;
Can be used in cell culture, protein purification, RNA preparation processes, etc.;
Pharmaceutical grade without registration.
HEPES buffer solution has the characteristics of being non-toxic to cells, unable to cross biofilms easily and does not interfere with biochemical reactions within cells;
Relevant studies have shown that using HEPES solution as the buffer system is beneficial to the coupling reaction of peptides on the surface of gold nanoparticles;
Relevant experiments on the impact of magnesium biodegradation show that HEPES can improve the biodegradability of magnesium and inhibit the formation of magnesium insoluble salt layers.
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